Fluorescent lipid staining
WebWith its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other … WebApr 21, 2024 · To overcome these limitations, we developed three novel extracellular vesicular-membrane-binding fluorescent probes-Mem dye-Green, Mem dye-Red, and Mem dye-Deep Red-for monitoring EV uptake into cells. The dyes contain a cyanine group as a fluorescent scaffold and amphiphilic moieties on the cyanine.
Fluorescent lipid staining
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WebApr 13, 2024 · The fluorescence was monitored at 650–750 nm. Lyso-Green was excited at 552 nm, and the fluorescence was monitored at 580–620 nm. Mito-Green was excited at 488 nm, and the fluorescence was monitored at 500–530 nm. BODIPY was excited at 488 nm, and the fluorescence was monitored at 490–590 nm. WebApr 14, 2024 · FDR and NES from 1000 permutations; (c) Mean fluorescence intensity at 510 nm of Bodipy 581/591 C11, which shows level of lipid peroxidation, in NT gRNA and MPI gRNA5 MOLM13 cells treated with ...
WebApr 7, 2024 · After staining with ROS-specific fluorescent dihydroethidium [DHE; dissolved in dimethyl sulfoxide (DMSO) to 2 mg / mL and diluted with PBS at a ratio of 1:500] dye (D7008; Sigma) at 37°C for 30 min and three rounds of PBS washes on a decolorizing shaker (5 min each), samples were incubated with 4′,6-diamidino-2-phenylindole (DAPI; …
WebWith its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids. We recommend dissolving in high-quality anhydrous DMSO to attain a stock concentration of 1 to 10 mM. For Research Use Only. Not for use in diagnostic procedures. WebMar 24, 2024 · Preparation of fluorescent lipid peroxidation reporter reagent Timing: 10 min This protocol uses BODIPY™ 581/591 C11 (Life Technologies, D3861) as the reporter for lipid peroxidation. BODIPY-C11 can be used to detect reactive oxygen species (ROS) in lipophilic environments such as cellular membranes.
WebAug 17, 2016 · Detailed protocols for some of the most popular and efficient contrasting methods are provided. Lipid Droplets (LDs), also known as lipid bodies, oil bodies or …
WebThe best method for combining visualization of intracellular lipid droplets with indirect immunofluorescent detection of lipid droplet-associated proteins is described. Techniques for sample fixation and permeabilization must be chosen carefully to avoid alterations to lipid droplet morphology. flower shop dubaiWebIn order to have the maximum and stable NR fluorescence, it is necessary to scan the excitation/emission wavelengths for up to a 40-min of incubation time. We outline a five-step method to customize the Nile Red protocol to a specific strain: 1) Evaluate the strain's suitability by checking for the presence of neutral lipid, 2) Select of the ... green bay fencing companiesWebMar 12, 2015 · Staining lipid droplets with BODIPY 505/515 is mainly performed by flow cytometer, which avoids the fluorescence background of the medium and explains the … green bay ferryWebDec 8, 2024 · The staining of lipids in algae cells with B odipy dyes is much less studied compared to Nile red; therefore, a complex of issues concerning staining details and … flower shop dunblaneWebWhile it is widely accepted that Nile Red staining yields unreliable results for detecting lipid structures in live C. elegansdue to strong interference of autofluorescence and non-specific staining signals, our results show that simple FLIM phasor analysis can effectively separate those signals and is capable of differentiating the non-polar … green bay ferry serviceWebFeb 1, 2024 · Indeed, the last decade has enriched the toolbox of general lipid stains, fluorescent lipid analogues, sugar-binding lectins, and protein-specific antibodies enabling the first rationally designed plasma membrane-specific dyes. Still, multiple challenges exist, and the unique properties of each dye must be considered when selecting a staining ... flower shop dundee miWebLive/dead cell staining. After mild photothermal treatment, the cells were stained by Calcein-AM/PI solution for 0.5 h under dark conditions and then were observed from fluorescence microscopy. Live cells presented green fluorescence, and dead cells showed red staining. The excitation wavelengths were 470 and 560 nm, respectively. green bay fencing