Tīmeklis2006. gada 21. febr. · The high-molecular-weight (HMW) subunits of glutenin from about 185 varieties were fractionated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). About 20 different, major subunits were distinguished by this technique although each variety contained, with only a few exceptions, between … Tīmeklis2024. gada 1. marts · We attempted to apply the common multiplex PCR system involving two groups of specific primers for two non-homologous genes, such as Fhb1 / Fhb7 for Fusarium head blight (FHB) resistance and Fhb1 / Pm21 for resistance to FHB and Powdery mildew [ 8, 10, 33 ].
High-density mapping of the major FHB resistance gene Fhb7
Tīmeklis2015. gada 29. jūl. · Fhb7 was mapped in a 1.7 cM interval that was flanked by molecular markers XsdauK66 and Xcfa2240 with SSR, diversity arrays technology, … Tīmeklis2024. gada 3. febr. · Seven FHB resistance genes have been identified and have been designated as Fhb1-Fhb7 so far, of which, Fhb1 and Fhb2 are derived from Sumai 3 … mangiafico
Theoretical and Applied Genetics Volume 135, issue 12 - Springer
Tīmeklis2024. gada 1. febr. · Zhengmai 9023 allele was predominant at Qfhb.2DS and Yangmai 158 allele was predominant at Qfhb.7D in the panel, indicating that markers for Qfhb.2DS and Qfhb.7D are likely polymorphic when their donors are crossed to most parents from the Middle and Lower Valleys of the Yangtze River in China. Tīmeklis2024. gada 21. okt. · Furthermore, individuals with favorable alleles at the four loci Fhb1, Qfhb.nc-2B.1 ( Q2B.1 ), Q7D.1, and Q7D.2 showed better FDK and DON scores (but not INC, SEV, and FHBdx) compared with other allelic combinations. Tīmeklis2024. gada 9. aug. · The 2846-bp Fhb7 homolog in the line CS-7EL and the probe 7EL-1 were labeled with Alexa Fluor-488-5-dUTP. The labeled probes were dissolved in 2 × SSC and 1× TE, dropped to the chromosome spreads. The slides were covered with a plastic sheet and denatured by heating at 100 °C for 5 min. mangiafico floridia